Chromatography and Membrane Filtration Quizzes for Bioprocess Engineering

Question 1

What is the primary mechanism of separation in gel filtration chromatography?

Accepted Answer

Size difference

Answer

Charge difference

Answer

Molecular weight difference

Answer

Hydrophobicity difference

Question 2

What is the key advantage of using hydrophobic interaction chromatography (HIC) in bioprocess engineering?

Accepted Answer

Mild elution conditions

Answer

High selectivity for proteins

Answer

Low cost

Answer

Scalability

Question 3

What is the major advantage of using ion exchange chromatography in bioprocess engineering?

Accepted Answer

High selectivity for charged molecules

Answer

Low cost

Answer

Ease of scale-up

Answer

Wide range of applications

Question 4

What is the role of a stationary phase in chromatography?

Accepted Answer

It provides a surface for interaction with the sample

Answer

It separates the sample components based on their affinity

Answer

It acts as a filter to remove impurities

Answer

It generates a signal that is detected by the detector

Question 5

Chromatography separates molecules based on their differential migration through a stationary phase using a mobile phase. Which of the following techniques employs this principle?

Accepted Answer

Chromatography

Answer

Centrifugation

Answer

Filtration

Answer

Crystallization

Question 6

In ion exchange chromatography, the stationary phase contains charged groups that interact with:

Accepted Answer

Negatively charged molecules

Answer

Neutral molecules

Answer

Positively charged molecules

Answer

Molecules with hydrophobic groups

Question 7

In size exclusion chromatography, the separation of molecules is based on their:

Accepted Answer

Size and shape

Answer

Charge

Answer

Hydrophobicity

Answer

Molecular weight

Question 8

Which of the following is NOT a type of chromatography?

Accepted Answer

Capillary electrophoresis

Answer

Gas chromatography

Answer

Liquid chromatography

Answer

Thin-layer chromatography

Question 9

In tangential flow filtration, the feed stream is:

Accepted Answer

Passed parallel to a membrane

Answer

Passed perpendicularly through a membrane

Answer

Forced through a membrane under high pressure

Answer

Separated into two streams based on particle size

Question 10

During the scale-up of chromatography processes from lab to production scale, which factor is essential for preserving separation effectiveness?

Accepted Answer

Consistent linear flow rate

Answer

Column diameter increase in proportion

Answer

Identical bed height across columns

Question 11

Which chromatography technique effectively separates proteins based on their size?

Accepted Answer

Size-exclusion chromatography

Answer

Ion-exchange chromatography

Answer

Affinity chromatography

Answer

Hydrophobic interaction chromatography

Question 12

In the membrane filtration process, what drives the separation?

Accepted Answer

Pressure gradient

Answer

Gravity

Answer

Electrical potential

Answer

Concentration gradient

Question 13

Which membrane pore structure allows the passage of water molecules and ions only?

Accepted Answer

Reverse osmosis

Answer

Microfiltration

Answer

Ultrafiltration

Answer

Nanofiltration

Question 14

In affinity chromatography, the stationary phase is modified with a ligand that specifically binds to the:

Accepted Answer

Affinity for the target molecule

Answer

Charge

Answer

Hydrophobicity

Answer

Molecular weight

Question 15

During ultrafiltration, the membrane retains molecules with a molecular weight:

Accepted Answer

Between 1 kDa and 100 kDa

Answer

Higher than 100 kDa

Answer

Less than 1 kDa

Answer

GreaterThan 1000 Da

Question 16

To determine the molecular weight of a protein separated by size-exclusion chromatography, which technique is appropriate?

Accepted Answer

Gel filtration calibration curve

Answer

SDS-PAGE

Answer

Western blotting

Answer

Isoelectric focusing

Question 17

In Size Exclusion Chromatography (SEC), what is the primary factor determining the separation of molecules?

Accepted Answer

Molecular size

Answer

Molecular charge

Answer

Molecular shape

Answer

Molecular affinity

Question 18

What is the primary purpose of using a guard column in chromatography?

Accepted Answer

To safeguard the analytical column from contaminants

Answer

To increase the sample loading capacity

Answer

To improve the resolution of the separation

Answer

To reduce backpressure

Question 19

Which of the following is not a type of stationary phase utilized in chromatography?

Accepted Answer

Filtration

Answer

Reversed phase

Answer

Ion exchange

Answer

Affinity

Question 20

What is the key difference between microfiltration and ultrafiltration?

Accepted Answer

Pore size

Answer

Membrane material

Answer

Filtration pressure

Answer

Flow rate

Question 21

Which of the following limitations is associated with membrane filtration?

Accepted Answer

Membrane fouling

Answer

High energy consumption

Answer

Low product recovery

Answer

Difficult scale-up

Question 22

What is the primary application of biochromatography in the field of bioprocess engineering?

Accepted Answer

Purification of biopharmaceuticals

Answer

Analysis of biomolecules

Answer

Separation of various cell types

Answer

Sterilization of bioprocess equipment